Ancient Breakthrough: Scientists Extract 6 Million-Year-Old DNA Fragments from Fossilized Turtle

Researchers excavate the 6 million-year-old fossil remains of a sea turtle of the genus Lepidochelys near La Pina along the Caribbean coast of Panama in this handout photograph taken in 2015 and obtained by Reuters on Sept. 28, 2023. (Carlos de Gracia/Handout via Reuters)

The fossilized DNA remains of an ancient sea turtle dating back 6 million years ago were discovered during excavations in the Caribbean region, researchers said Thursday.

The researchers said some bone cells, called osteocytes, were exquisitely preserved in the fossil, which was excavated along Panama’s Caribbean coast in 2015. The fossil is partial, with a relatively complete carapace – the turtle’s shell – but not the rest of the skeleton. The turtle would have been about a foot (30 cm) long when alive, they said.

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In some of the osteocytes, the cell nuclei were preserved and reacted to a chemical solution that allowed the researchers to recognize the presence of remnants of DNA, the molecule that carries genetic information for an organism’s development and functioning, said paleontologist Edwin Cadena, lead author of the study published in the Journal of Vertebrate Paleontology.

“I want to point out that we did not extract DNA, we only were able to recognize the presence of DNA traces in the nuclei,” added Cadena, of Universidad del Rosario in Bogota and the Smithsonian Tropical Research Institute.

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DNA is quite perishable, though in the right conditions, it has been preserved in some ancient remains. Researchers last year reported the discovery of DNA from animals, plants and microbes dating to about 2 million years ago from sediment at Greenland’s remote northernmost point.

Cadena said the only older vertebrate fossils than the newly described turtle to have been found with similar DNA remnants were of two dinosaurs – Tyrannosaurus, which lived about 66 million years ago, and Brachylophosaurus, which lived about 78 million years ago. Cadena said DNA remnants also have been reported in insects dating to tens of millions of years ago.

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The turtle is from the same genus – Lepidochelys – as two of the world’s seven living species of sea turtles – the Kemp’s ridley, the world’s smallest sea turtle, and the olive ridley, Cadena said. Kemp’s ridley, with a triangular-shaped head and a slightly hooked beak, is primarily found in the Gulf of Mexico. The olive ridley, which closely resembles Kemp’s ridley, has a larger distribution, primarily found in the tropical regions of the Pacific, Indian and Atlantic oceans.

The fossil represents the oldest-known member of Lepidochelys and helps to shed light on the poorly understood evolutionary history of this genus, the researchers said. They did not identify it by species because the remains were too incomplete, Cadena said.

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“Each fossil, each fossil site has specific conditions of preservation that in some cases could have favored preservation of original biomolecular remains such as proteins and DNA,” Cadena said.

“Maybe in the future and with more studies of this kind, we could be able at some point to sequence very small pieces of DNA and to infer things about their close relatives or involve that information in a broader molecular evolutionary study,” Cadena added.

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